Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies

Ahmad-Raus, Raha and Ali, Abdul Manaf and Tan, Wen Siang and Mohd. Salleh, Hamzah and Majid, Eshaghi and Yusoff, Khatijah (2009) Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies. Research in Veterinary Science, 86 (1). pp. 174-182. ISSN 0034-5288

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A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli. One of the antigenic sites was located at the C-terminal end (residues 441 to 489) of the NP protein. Two other antigenic sites were located within the N-terminal end (residues 26–121 and 122–375). This study demonstrates that the N- and C-terminal ends of the NP proteins are responsible in eliciting immune response, thus it is most likely that these ends are exposed on the NP.

Item Type:Article
Keyword:Monoclonal antibody; Nucleocapsid; Antigenic sites; Newcastle disease virus
Subject:Monoclonal antibody
Subject:Antibodies, Monoclonal - Analysis
Subject:Newcastle disease virus
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Elsevier Ltd.
DOI Number:10.1016/j.rvsc.2008.05.013
ID Code:15482
Deposited By: Nida Hidayati Ghazali
Deposited On:01 Aug 2012 04:38
Last Modified:23 Oct 2012 04:52

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