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Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica


Citation

Abd. Shukor, Mohd. Yunus and Ali Hassan, Nor Ayshah Alia and Jusoh, Arif Zaidi and Nagar, Perumal and Shamaan, Nor Aripin and Mac Cormack, Walter Patricio and Syed, Mohd Arif (2009) Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica. Journal of Environmental Biology, 30 (1). pp. 1-6. ISSN 0254-8704; ESSN: 2394-0379

Abstract

A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Triveni Enterprises
Keywords: Antarctica; Diesel degradation; Pseudomonas
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 25 Jul 2012 03:53
Last Modified: 27 Mar 2018 08:56
URI: http://psasir.upm.edu.my/id/eprint/15320
Statistic Details: View Download Statistic

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