Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica

Abd. Shukor , Mohd. Yunus and Hassan, N.A.A. and Jusoh, A.Z. and Perumal, N. and Shamaan, Nor Aripin and MacCormack, W.P. and Syed, Mohd. Arif (2009) Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica. Journal of Environmental Biology., 30 (1). pp. 1-6. ISSN 0254-8704

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Abstract

A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium.

Item Type:Article
Keyword:Antarctica; Diesel degradation; Pseudomonas
Subject:Antarctica
Subject:Pseudomonas
Subject:Bacteria - Identification
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Academy of Environmental Biology
ID Code:15320
Deposited By: Nida Hidayati Ghazali
Deposited On:25 Jul 2012 03:53
Last Modified:25 Oct 2012 04:00

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