Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.

Shafiquzzaman, Siddiquee and Tan, Soon Guan and Yusuf, Umi Kalsom (2010) Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates. Journal of Microbiology and Biotechnology, 20 (9). pp. 1266-1275. ISSN 1017-7825

Full text not available from this repository.


Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderm a species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species.

Item Type:Article
Keyword:Trichoderma harzianum; Isozyme and allozyme activities; Protein patterns; Cluster analysis.
Subject:Isoenzymes - Analysis.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Korean Society for Microbiology and Biotechnology (KMB)
DOI Number:doi: 10.4014/jmb.1004.04029
ID Code:15318
Deposited By: Nida Hidayati Ghazali
Deposited On:01 Aug 2012 01:31
Last Modified:01 Aug 2012 01:31

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 01 Aug 2012 01:31.

View statistics for "Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates."

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.