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Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.

Shafiquzzaman, Siddiquee , Tan, Soon Guan and Yusuf, Umi Kalsom (2010) Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates. Journal of Microbiology and Biotechnology, 20 (9). pp. 1266-1275. ISSN 1017-7825

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Abstract

Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderm a species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species.

Item Type:Article
Keyword:Trichoderma harzianum; Isozyme and allozyme activities; Protein patterns; Cluster analysis.
Subject:Trichoderma.
Subject:Isoenzymes.
Subject:Isoenzymes - Analysis.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Korean Society for Microbiology and Biotechnology (KMB)
DOI Number:doi: 10.4014/jmb.1004.04029
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4014/jmb.1004.04029
ID Code:15318
Deposited By: Nida Hidayati Ghazali
Deposited On:01 Aug 2012 01:31
Last Modified:01 Aug 2012 01:31

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