Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus

Abd. Shukor , Mohd. Yunus and Rahman, MF and Suhaili, Zarizal and Shamaan, Nor Aripin and Syed, Mohd. Arif (2010) Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus. Folia microbiologica, 55 (2). pp. 137-143. ISSN 0015-5632

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A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction.

Item Type:Article
Keyword:Molybdenum reduction; Acinetobacter
Subject:Molybdenum - Analysis
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Elsevier B.V.
DOI Number:DOI: 10.1007/s12223-010-0021-x
ID Code:14999
Deposited By: Nida Hidayati Ghazali
Deposited On:01 Aug 2012 07:40
Last Modified:18 Oct 2012 09:00

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