Chaibakhsh, Naz and Abdul Rahman, Mohd Basyaruddin and Basri , Mahiran and Salleh, Abu Bakar and Raja Othman, Raja Nor Izawati (2009) Effect of alcohol chain length on the optimum conditions for lipase-catalysed synthesis of adipate esters. Biocatalysis and Biotransformation, 27 (5-6). pp. 303-308. ISSN 1024-2422
Full text not available from this repository.
Immobilized Candida antarctica lipase B, Novozym® 435, was used in the esterification of adipic acid and alcohols with different chain lengths (C1–C18). Optimum conditions for the synthesis of adipate esters were obtained using response surface methodology (RSM) with respect to important reaction parameters including time, temperature, substrate molar ratio and amount of enzyme. Alcohol chain length specificity of the enzyme in the synthesis of adipate esters was also determined. Minimum reaction time (215 min) for achieving maximum ester yield was obtained for butyl alcohol. Methanol required an increased time (358 min) and enzyme amount (10.2%, w/w) for attaining maximum yield. The maximum required temperature and time of 65°C and 523 min, respectively, were obtained for the synthesis of dioctadecyl adipate. The results demonstrate that alcohol chain length is a determining parameter in optimization of the lipase-catalyzed synthesis of adipate esters. Reactions under optimized conditions yielded a high percentage of esterification (>97%). The optimum conditions can be used to scale up the process.
|Keyword:||Esterification; Lipase; Adipate ester; Specificity; Optimization; Response surface methodology|
|Faculty or Institute:||Faculty of Biotechnology and Biomolecular Sciences|
|DOI Number:||DOI: 10.3109/10242420903207584|
|Deposited By:||Nida Hidayati Ghazali|
|Deposited On:||29 Aug 2012 01:10|
|Last Modified:||19 Dec 2012 06:47|
Repository Staff Only: item control page
Document Download Statistics
This item has been downloaded for since 29 Aug 2012 01:10.