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Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor

Abd. Aziz, Suraini and Hassan, Mohd Ali and Fernandez, Christine C. and Md Salleh, Madihah and Md Illias, Rosli (2008) Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor. Applied Biochemistry and Biotechnology, 150 (2). pp. 193-204. ISSN 0273-2289

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Abstract

Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, chitinase-producing fungi have been extensively studied as biocontrol agents. Locally isolated Trichoderma virens UKM1 was used in this study. The effect of agitation and aeration rates using colloidal chitin as control substrate in a 2-l stirred tank reactor gave the best agitation and aeration rates at 200 rpm and 0.33 vvm with 4.1 U/l per hour and 5.97 U/l per hour of maximum volumetric chitinase activity obtained, respectively. Microscopic observations showed shear sensitivity at higher agitation rate of the above system. The oxygen uptake rate during the highest chitinase productivity obtained using sun-dried ground shrimp waste of 1.74 mg of dissolved oxygen per gram of fungal biomass per hour at the kappaL a of 8.34 per hour.

Item Type:Article
Keyword:Agitation; Aeration; Chitinase; Trichoderma virens UKM1
Subject:Chitinase
Subject:Aeration tanks - Testing
Subject:Reactors
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Humana Press
ID Code:14203
Deposited By: Nida Hidayati Ghazali
Deposited On:29 Aug 2012 09:14
Last Modified:18 Oct 2012 15:52

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