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Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia


Citation

A. Rashid, Fairuz Ridzlan and Bahaman, Abdul Rani and Bejo, Siti Khairani and Mutalib, Abdul Rahim (2010) Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia. Tropical Biomedicine, 27 (3). pp. 632-638. ISSN 0127-5720

Abstract

Leptospirosis is recognized as one of the important zoonotic diseases in the world including Malaysia. A total of 145 soil and water samples were collected from selected National Service Training Centres (NSTC) in Kelantan and Terengganu. The samples were inoculated into modified semisolid Ellinghausen McCullough Johnson Harris (EMJH) medium, incubated at room temperature for 1 month and examined under the dark-field microscope. Positive growth of the leptospiral isolates were then confirmed with 8-Azaguanine Test, Polymerase Chain Reaction (PCR) assay and Microscopic Agglutination Test (MAT). Fifteen cultures (10.34%) exhibited positive growths which were seen under dark field microscope whilst only 20% (3/15) were confirmed as pathogenic species. based on 8-Azaguanine Test and PCR. Serological identification of the isolates with MAT showed that hebdomadis was the dominant serovar in Terengganu. Pathogenic leptospires can be detected in Malaysian environment and this has the potential to cause an outbreak. Therefore, precautionary steps against leptospirosis should be taken by camp authorities to ensure the safety of trainees.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Malaysian Society of Parasitology and Tropical Medicine
Keywords: Leptospires; Environment; Kelantan; Terengganu
Depositing User: Azizan Arshad
Date Deposited: 09 Dec 2013 03:56
Last Modified: 10 Jan 2017 05:54
URI: http://psasir.upm.edu.my/id/eprint/13936
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