UPM Institutional Repository

Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens


Citation

Ramasamy, Kalavathy and Abdullah, Norhani and Wong, Clemente Michael Vui Ling and Chinna, Karuthan and Ho, Yin Wan (2010) Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens. Journal of the Science of Food and Agriculture, 90 (1). pp. 65-69. ISSN 0022-5142; ESSN: 1097-0010

Abstract

BACKGROUND: Bile salt deconjugation by Lactobacillus strains is often closely linked to bile tolerance and survival of the strains in the gut and lowering of cholesterol in the host. The present study investigated the deconjugation of bile salts and removal of cholesterol by 12 Lactobacillus strains in vitro. The 12 strains were previously isolated from the gastrointestinal tract of chickens. RESULTS: The 12 Lactobacillus strains could deconjugate sodium glycocholate (GCA, 16.87-100%) and sodium taurocholate (TCA, 1.69-57.43%) bile salts to varying degrees, with all strains except L. salivarius I 24 having a higher affinity for GCA. The 12 Lactobacillus strains also showed significant (P < 0.05) differences in their ability to remove cholesterol from the growth medium (26.74-85.41%). Significant (P < 0.05) correlations were observed between cholesterol removal and deconjugation of TCA (r = 0.83) among the L. reuteri strains (C1, C10 and C16) and between cholesterol removal and deconjugation of TCA (r = 0.38) and GCA (r = 0.70) among the L. brevis strains (I 12, I 23, I 25, I 211 and I 218). In contrast, although L. gallinarum I 16 and I 26 and L. panis C 17 showed high deconjugating activity, there was no correlation between cholesterol removal and deconjugation of bile salts in these strains. CONCLUSION: The results showed that the 12 Lactobacillus strains were able to deconjugate bile salts and remove cholesterol in vitro, but not all strains with high deconjugating activity removed cholesterol effectively.


Download File

[img]
Preview
PDF (Abstract)
Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens.pdf

Download (84kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1002/jsfa.3780
Publisher: Society of Chemical Industry
Keywords: Lactobacillus; Probiotics; Bile salts; BSH; Deconjugation; Cholesterol
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 08 Jun 2015 03:50
Last Modified: 27 Nov 2019 06:48
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jsfa.3780
URI: http://psasir.upm.edu.my/id/eprint/13393
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item