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Generation, fractionation, and characterization of iron-chelating protein hydrolysate from palm kernel cake proteins

Zarei, Mohammad and Ghanbari, Rahele and Tajabadi, Naser and Abdul Hamid, Azizah and Abu Bakar, Fatimah and Saari, Nazamid (2016) Generation, fractionation, and characterization of iron-chelating protein hydrolysate from palm kernel cake proteins. Journal of Food Science, 81 (2). C341-C347. ISSN 0022-1147; ESSN: 1750-3841

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Palm kernel cake protein was hydrolyzed with different proteases namely papain, bromelain, subtilisin, flavourzyme, trypsin, chymotrypsin, and pepsin to generate different protein hydrolysates. Peptide content and iron-chelating activity of each hydrolysate were evaluated using O-phthaldialdehyde-based spectrophotometric method and ferrozine-based colorimetric assay, respectively. The results revealed a positive correlation between peptide contents and iron-chelating activities of the protein hydrolysates. Protein hydrolysate generated by papain exhibited the highest peptide content of 10.5 mM and highest iron-chelating activity of 64.8% compared with the other hydrolysates. Profiling of the papain-generated hydrolysate by reverse phase high performance liquid chromatography fractionation indicated a direct association between peptide content and iron-chelating activity in most of the fractions. Further fractionation using isoelectric focusing also revealed that protein hydrolysate with basic and neutral isoelectric point (pI) had the highest iron-chelating activity, although a few fractions in the acidic range also exhibited good metal chelating potential. After identification and synthesis of papain-generated peptides, GGIF and YLLLK showed among the highest iron-chelating activities of 56% and 53%, whereas their IC50 were 1.4 and 0.2 μM, respectively.

Item Type:Article
Keyword:Chelating activity; Palm kernel cake; Peptide; Protein hydrolysate
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Institute of Food Technologists
DOI Number:10.1111/1750-3841.13200
ID Code:12542
Deposited By: Nabilah Mustapa
Deposited On:01 Jun 2011 16:43
Last Modified:08 Jun 2016 09:34

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