Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers

Darvari, Fatemeh Mahdavi and Meon, Sariah and Abdullah, Mohd Puad and Mahmood, Maziah (2010) Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers. African Journal of Biotechnology, 9 (16). pp. 2360-2366. ISSN 1684–5315

[img] PDF - Updated Version
858Kb

Abstract

Male inflorescences have potential to be used as explants for rapid micropropagation of Musa sp. The male flowers of four banana cultivars, namely ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Abu’ belonging to three genome types in Musa (AAA, AAB, and ABB), were cultured onto Murashige and Skoog (MS) medium which was supplemented with 1 mg/L of TDZ, BAP, Kin, 2-ip and Zea. The number of shoots was found to significantly increase in both TDZ and BAP treatments, as compared to other cytokinins. TDZ at 0.4, 0.6 and 0.8 mg/L, in particular, appeared to be optimum for shoot induction in ‘Berangan- AAA’, ‘Rastali-AAB’ and ‘Nangka-AAB’ and ‘Abu-ABB’, respectively. However, all the cultivars showed their highest response to regeneration at 8 mg/L of BAP. After the initiation of the explants onto the MS media for all the cultivars, the highest number of cauliflower-like bodies’ (CLBs) clusters was observed at two months of culture. The number of induced ‘CLBs’ cluster is dependent on the size of male buds. Male inflorescences with the size of 20 mm were found to induce more ‘CLBs’ clusters. Meanwhile, the number of shoots produced is dependent on both the cytokinins and cultivars used.

Item Type:Article
Keyword:Proliferation rate, Musa, cytokinin, male flower, male inflorescence, micropropagation
Faculty or Institute:Faculty of Agriculture
Publisher:Academic Journals
ID Code:12399
Deposited By: Norhazura Hamzah
Deposited On:18 May 2011 05:37
Last Modified:27 May 2013 07:52

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 18 May 2011 05:37.

View statistics for "Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.