Genetic Variation of Selected Camellia sinensis (Cultivated Tea)Varieties in Malaysia Based on Random Amplified Microsatellite(RAMs) Markers

Latip, S. N. H. and Muhamad Awang, Rita and Manjeri, G. and Tan, Soon Guan (2010) Genetic Variation of Selected Camellia sinensis (Cultivated Tea)Varieties in Malaysia Based on Random Amplified Microsatellite(RAMs) Markers. Pertanika Journal of Tropical Agricultural Science, 33 (2). pp. 259-267. ISSN 1511-3701

[img] PDF - Published Version
1217Kb

Abstract

Studies on the genetic variation among Camellia sinensis L. varieties (cultivated tea) in Malaysia were conducted by using RAMs markers. Six varieties were selected from Sungai Palas Boh Estate, Cameron Highlands and nine varieties were selected from Bukit Cheeding Boh Estate, Banting. These tea varieties were classified as resistant, intermediate, or susceptible varieties based on the level of infestation by the mosquito bug, Helopeltis theivora. DNA was extracted from the leaves of 225 individuals belonging to different varieties from the two populations. Four RAMs primers were used to evaluate the genetic variation in 15 varieties of tea. Distances were calculated based on Nei and Li’s (1979) similarity coefficients using the data from the RAMs markers. A cluster analysis employing UPGMA was done and the dendrogram grouped the tea varieties into two clusters with intermediate variety grouping and the resistant (the first cluster) or susceptible (the second cluster) varieties. The first cluster consisted of all the varieties from Cameron Highlands, except for BC223 (resistant) and 63/14 (resistant) from the Banting population, while the other clusters consisted of all the varieties from the Banting population, except for BC196 (resistant) from the Cameron Highlands population. The dendrogram showed that the genetic differences were based on the populations’ geographical distributions and partially based on their resistance towards attack by H. theivora.

Item Type:Article
Keyword:Genetic variation, tea, RAMs, resistant, susceptible varieties
Faculty or Institute:Faculty of Agriculture
Publisher:Universiti Putra Malaysia Press
ID Code:12388
Deposited By: kmportal
Deposited On:18 May 2011 05:28
Last Modified:27 May 2013 07:51

Repository Staff Only: item control page


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.