Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat

Chandrasiri, Alawattage Don Nimal (1997) Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat. PhD thesis, Universiti Pertanian Malaysia.

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Abstract

A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to heparin could be used for sperm capacitation. The acrosome reaction was tested by a dual staining technique (Experiment 1 ) and the zona free hamster penetration test (Experiment 2). In vitro maturation o f goat oocytes and fertilisation were assessed by the number of zygotes cleaving beyond the 2-cell stage (Experiment 3). Freshly ejaculated semen from two fertile bucks were used for sperm capacitation using two defined media (DM-H and DM-Ca). Heparin and S-ACT-l were tested at concentrations of 10, 20, 50 and 100 ug/ml. After incubating for 15 min. at 39° C with 5 % CO2 in air under maximum humidity, samples were stained with trypan blue and Giemsa stain (dual stain). The results indicated that the sperm treated with heparin and S-ACT - 1 showed similar staining characteristics.

Item Type:Thesis (PhD)
Subject:Goats - Fertility
Subject:Fertilization in vitro
Chairman Supervisor:Professor Tengku Azmi bin Tengku Ibrahim, PhD
Call Number:FPV 1997 6
Faculty or Institute:Faculty of Veterinary Medicine
ID Code:12327
Deposited By: Mohd Nezeri Mohamad
Deposited On:13 Jul 2011 01:22
Last Modified:12 Sep 2011 01:34

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