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Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin


Citation

Cheow, Loretta Marie (1992) Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin. Masters thesis, Universiti Pertanian Malaysia.

Abstract / Synopsis

This research was carried out to investigate two isolates of bovine herpes virus I (BHV -l) isolated from buffaloes at Universiti Pertanian Malaysia , Serdang , Selangor Darul Ehsan, and compare them to a reference cattle strain of BHV- l ( Cooper strain) . The cytopathogenic and serologic properties of the three virus isolated (Buffalo respiratory , Buffalo genital , and the Cooper strain) were first studied. The cytopathogenicity of the three BHV-1 isolates was investigated using Acridine Orange staining (to compare localization of DNA) , Hematoxylin and Eosin staining (to compare morphologic changes induced in cell culture), the indirect Immunoperoxidase ( lIP) staining method ( to compare localization o f the virus antigen ) , plaque assay, and Negative Contrast Electron Microscopy.


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Additional Metadata

Item Type: Thesis (Masters)
Call Number: FPV 1992 3
Chairman Supervisor: Professor Abdul Latif Ibrahim
Divisions: Faculty of Veterinary Medicine
Depositing User: Mohd Nezeri Mohamad
Date Deposited: 13 Jul 2011 12:17
Last Modified: 13 Jul 2011 12:17
URI: http://psasir.upm.edu.my/id/eprint/12299
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