Cheow, Loretta Marie (1992) Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin. Masters thesis, Universiti Putra Malaysia.
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Abstract
This research was carried out to investigate two isolates of bovine herpes virus I (BHV -l) isolated from buffaloes at Universiti Pertanian Malaysia , Serdang , Selangor Darul Ehsan, and compare them to a reference cattle strain of BHV- l ( Cooper strain) . The cytopathogenic and serologic properties of the three virus isolated (Buffalo respiratory , Buffalo genital , and the Cooper strain) were first studied. The cytopathogenicity of the three BHV-1 isolates was investigated using Acridine Orange staining (to compare localization of DNA) , Hematoxylin and Eosin staining (to compare morphologic changes induced in cell culture), the indirect Immunoperoxidase ( lIP) staining method ( to compare localization o f the virus antigen ) , plaque assay, and Negative Contrast Electron Microscopy.
| Item Type: | Thesis (Masters) |
|---|---|
| Chairman Supervisor: | Professor Abdul Latif Ibrahim |
| Call Number: | FPV 1992 3 |
| Faculty or Institute: | Faculty of Veterinary Medicine |
| ID Code: | 12299 |
| Deposited By: | Mohd Nezeri Mohamad |
| Deposited On: | 13 Jul 2011 12:17 |
| Last Modified: | 13 Jul 2011 12:17 |
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