Antimicrobial Resistance Of Escherichia Coli Isolates In Pig Farm Workers, Normal And Diarrhoeic Pigs

Abstract

The enumeration of coliforms in faeces can be per formed by both the spread plate method or Most Probable Number ( MPN ) techniques , although MPN total coliform counts are significantly higher ( P<0.05 ) . In so lid agar media enumeration , MacConkey , Desoxycholate , Eosin-Methylene Blue and ENDO agars are suitable media for the enumeration of coliforms . The correlation coefficients of counts among media and technique is high . The mean colony forming unit ( CFU ) counts per gram for coliform in faeces varied from 2 . 6-6 . 4 X 107 and 1 . 8-2 . 7 X 108 for the pigs and form workers tested respectively . Random identification of 291 colonies on MacConkey agar on the basis of frequency of occurrence of numbers of lactose and nonlactose fermenters yielded 94 . 2 % Escherichia coli (E.coli).The other genera isolated includes Enterobacter , Klebsiella , Edwarsiella , Citrobacter and Serratia . About 8.2 % and 29.6 % of the E. coli isolates were non-lactose fermenters on MacConkey agar and did not show the characteristic green metallic sheen on Eosin- Methylene Blue agar respectively.