A Study On A Velogenic Viscerotropic Newscastle Disease Virus In Vitro And In Vivo

Lai, Chooi May (1985) A Study On A Velogenic Viscerotropic Newscastle Disease Virus In Vitro And In Vivo. PhD thesis, Universiti Pertanian Malaysia.

[img] PDF
1589Kb

Abstract

The velogenic viscerotropic Newcastle disease virus or the Asiatic strain has been considered the most virulent strain of Newcastle disease virus. It is commonly found in Southeast Asia and it has been known to cause 100\ mortality in susceptible flocks. In spite of this, very little research has been conducted on it as many countries prohibit the handling of this strain of virus. In view of this, a project has been undertaken at Universiti Pertanian Malaysia to study the biological properties, cytopathogenicity and morphogenesis of a locally isolated velogenic viscerotropic Newcastle disease virus and to determine its effects on the trachea of nonvaccinated and vaccinated chickens. The in vitro study has shown that this virus has a mean death time of 66 hours, and an intracerebral pathogenicity index of 1.90 . Polykaryocytosis is the principal form of cytopathic effect it produces in chick embryo fibroblasts and cells infected by it haemadsorp red blood cells. This virus plaques in cell culture. Negatively stained virus particles have diameters ranging from 100 to 600 nanometers. Electron microscopy demonstrated that the virus replicates in the cytoplasm of infected cells and aggregates of nucleocapsids are found in the cytoplasm. The virus matures at the cell membrane and is released by budding.

Item Type:Thesis (PhD)
Chairman Supervisor:Professor Abdul Latif bin Ibrahim
Call Number:FPV 1985 1
Faculty or Institute:Faculty of Veterinary Medicine
ID Code:12264
Deposited By: Mohd Nezeri Mohamad
Deposited On:13 Jul 2011 07:33
Last Modified:09 Sep 2011 02:33

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 13 Jul 2011 07:33.

View statistics for "A Study On A Velogenic Viscerotropic Newscastle Disease Virus In Vitro And In Vivo "


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.