Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost

Samsu Baharuddin, Azhari and Abd. Razak, Mohamad Nafis and Lim, Siong Hock and Ahmad, Mohd Najib and Abd. Aziz, Suraini and Abdul Rahman, Nor’ Aini and Md Shah, Umi Kalsom and Hassan, Mohd Ali and Kenji, Sakai and Yoshihito, Shirai (2010) Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost. American Journal of Applied Sciences, 7 (1). pp. 56-62. ISSN 1546-9239

Full text not available from this repository.

Abstract

Problems statement: Lack of information on locally isolated cellulase-producing bacterium in thermophilic compost using a mixture of Empty Fruit Bunch (EFB) and Palm Oil Mill Effluent (POME) as composting materials. Approach: The isolation of microbes from compost heap was conducted at day 7 of composting process where the mixture of composting materials consisted of 45.8% cellulose, 17.1% hemicellulose and 28.3% lignin content. The temperature, pH and moisture content of the composting pile at day 7 treatment were 58.3, 8.1 and 65.5°C, respectively. The morphological analysis of the isolated microbes was conducted using Scanning Electron Microscope (SEM) and Gram stain method. The congo red test was conducted in order to detect 1% CMC agar degradation activities. Total genomic DNAs were extracted from approximately 1.0 g of mixed compost and amplified by using PCR primers. The PCR product was sequent to identify the nearest relatives of 16S rRNA genes. The localization of bacteria chromosomes was determined by Fluorescence In Situ Hybridization (FISH) analysis. Results: Single isolated bacteria species was successfully isolated from Empty Fruit Bunch (EFB)-Palm Oil Mill Effluent (POME) compost at thermophilic stage. Restriction fragment length polymorphism profiles of the DNAs coding for the 16S rRNAs with the phylogenetic analysis showed that the isolated bacteria from EFB-POME thermophilic compost gave the highest homology (99%) with similarity to Geobacillus pallidus. The strain was spore forming bacteria and able to grow at 60°C with pH 7. Conclusion: Thermophilic bacteria strain, Geobacillus pallidus was successfully isolated from Empty Fruit Bunch (EFB) and Palm Oil Mil Effluent (POME) compost and characterized.

Item Type:Article
Keyword:Cellulase; Thermophilic bacteria; Composting; Empty fruit bunch; Palm oil mill effluent
Faculty or Institute:Faculty of Engineering
DOI Number:10.3844/ajassp.2010.56.62
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3844/ajassp.2010.56.62
ID Code:12103
Deposited By: Anas Yahaya
Deposited On:27 Apr 2011 01:07
Last Modified:03 Jul 2014 05:18

Repository Staff Only: Edit item detail


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.