Isolation And Characterization Of Phenylalanine Ammonia Lyase (Pal) And Other Defence Response Genes From Oil Palm (Elaies Guineensis Jacq.)

Hwang, Siaw San (2002) Isolation And Characterization Of Phenylalanine Ammonia Lyase (Pal) And Other Defence Response Genes From Oil Palm (Elaies Guineensis Jacq.). Masters thesis, Universiti Pertanian Malaysia.

[img] PDF
2104Kb

Abstract

Enhanced disease resistance and plant defense response against pathogen attack and environmental stresses have always been important targets of plant biotechnology. The activation of the defense response requires the recognition of an elicitor and the subsequent initiation of a signal transduction pathway, which then leads to the activation of defense genes and the production of phytoalexins. Plants respond to pathogen attack and environmental stimuli by activating a wide variety of defense reactions including transcriptional activation of genes involved in phenylpropanoid biosynthesis, accumulation of antimicrobial phytoalexins, and ethylene production. Phenylpropanoid metabolism is a plant specific pathway that leads to the production of secondary metabolites including isoflavonoid phytoalexins, lignin, flavonoid pigments and UV protectants such as furanocoumarin. Here, we report the isolation of several , different clones that are involved in the plant defense response by screening of oil palm zygotic embryo and suspension culture cDNA libraries.These clones include phenylalanine ammonia- lyase (PAL) (4-4A, II F I and 12B1), S-adenosylmethionine synthetase I (Adomet synthetase I) (11 AI), peroxidase (7 A2), chitinase III (4A3), calmodulin (3E2), and beta-ketoacyl-CoA synthase (KCS) (3G 1).

Item Type:Thesis (Masters)
Subject:Phenylalanine ammonia lyase
Subject:Palm oil
Chairman Supervisor:Tan Siang Hee, PhD
Call Number:FSMB 2002 7
Faculty or Institute:Faculty of Food Science and Technology
ID Code:11858
Deposited By: Mohd Nezeri Mohamad
Deposited On:19 Jul 2011 06:57
Last Modified:19 Jul 2011 06:57

Repository Staff Only: Edit item detail


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.