Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species

Juan Deanon, Albaladejo (1994) Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species. Masters thesis, Universiti Pertanian Malaysia.

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Abstract

Three virus particles were isolated in vitro and tentatively classified into three groupings , namely Herpesviridae, Birnaviridae and Iridoviridae . Virus groupings were based on the ultrastructural morphology and cytopathology observed . Distinct virus-like cytopathic effects were noted on four established fish cell lines viz . CHSE-214 , GCK-84 , EPC and TmB , utilized in the study . The virus - like particles , from pelleted infected cell cultures and clarified cell culture supernatant, were visualized under transmission electron microscopy .Virus titration utilizing BB cells for the Birnavirus - like isolate achieved a mean titre of 7.40 X 105 TC ID50/ml. Relatively high virus titres were also recorded from the other two isolants , with a mean virus titres of 7.16 X 105 TC ID50/ml for Herpesvirus - like and 6.4 6 'x 105 TCID50/ml for Iridovirus-like .

Item Type:Thesis (Masters)
Subject:Carp - Virus diseases
Subject:Tilapia - Virus diseases
Subject:Herpesvirus diseases in animals
Chairman Supervisor:Dr . Hassan Hj . Mohd.Daud
Call Number:FPSS 1994 4
Faculty or Institute:Faculty of Fisheries and Marine Science
ID Code:11797
Deposited By: Mohd Nezeri Mohamad
Deposited On:21 Jul 2011 00:56
Last Modified:10 Aug 2011 07:12

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