Effects of Progesterone Intravaginal Devices on Artificial Insemination, Oestrous Synchronization, Ovulation and Pregnancy Rate in Kedah-Kelantan Cattle
Khor, Chun Siah (2002) Effects of Progesterone Intravaginal Devices on Artificial Insemination, Oestrous Synchronization, Ovulation and Pregnancy Rate in Kedah-Kelantan Cattle. Masters thesis, Universiti Putra Malaysia.
The first experiment was conducted to evaluate the effect of various progestagen treatment methods in term of oestrous synchronization, ovulation and pregnancy rate using controlled internal drug-release (CIDR) device or progesterone-releasing intravaginal device (PRID) with prostaglandin F2ct (PGF2ct) injection on the day of CIDRIPRID removal. Twenty-four non-pregnant Kedah-Kelantan (KK) cows were randomly assigned to two groups: CIDR (n=12); PRID (n=12). Both group were given 12-day synchronization treatment and received an intramuscular injection of 25 mg PGF2ct on the day of device removal. The ovaries of all cows were examined by ultrasonography, using a 5 MHz probe, from one day after CIDR/PRID removal until ovulation. Blood samples were collected for determination of progesterone. The proportion of cows observed in oestrus was significantly higher in the CIDR group than PRID group (91.7% vs 58.3%, P<O.Ol). Pregnancy rate was also significantly higher in CIDR group than PRID group (27.3% vs 14.2%, P<0.05). Results from experiment indicated that CIDR is more efficient than PRID. The second experiment was to evaluate the effect of the length of progestagen treatment (12 day vs 7 day) on oestrous synchronization, ovulation and pregnancy rate using controlled internal drug-release device (CIDR) with PGF2a injection at the end of CIDR treatment. Sixteen adult non-pregnant KK cows were divided into two groups: long-term treated group (n=8); Short-term treated group (n=8), day of CIDR removal. All the cows were undergone transrectal ultrasonography, and blood samples were taken for hormone assay. Until 120 h after CIDR removal, the percentage of cows came into oestrus was significantly higher in long-termtreated group than short-term treated group (87.5%). The pregnancy rate was significantly higher in the short-term treated group than in long-term-treated group (40.0% vs 25.0%, follicle in long-term treated group attained a larger maximum diameter than in short-term treated group (P<0.05). The mean time of ovulation after device removal was 108 h in the short-term treated group and 88 ± 5.06 h in the long-tenn treated group; the length of time required for ovulation between two treatment were significantly different (P<0.05). The result showed that the 7-d CIDR+ PGF2a oestrous synchronization protocol rendered a better fertility in Kedah-Kelantan cows.
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