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Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85

Suryadi, Yadi (2001) Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85. Masters thesis, Universiti Putra Malaysia.

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Abstract

pBX6 is a recombinant plasmid containing a 3 kb fragment of insert D NA from Fibrobacter succinogenes S 85 genomic DNA which encodes xylanase activity. The recombinant plasmid pBX6 has shown xylanase activity when grown on RBB-xylan plates supplemented with ampicillin. Restriction mapping of the plasmid established the presence of single sites for EcoRI, Kpnl, Apal, Saci, EcoRV and Pstf. This work was focussed on nucleotide sequence determination of recombinant plasmid pBX6 containing a xyJanase gene from F. succinogenes S 85. The primer walking strategy was performed to complete manual sequencing, following the protocol cycle sequencing of Sanger dideoxy chain termination. Custom synthesis primer was designed and constructed from 3' end of the preceding sequence on both strands.

Item Type:Thesis (Masters)
Subject:Genetic recombination - Research
Chairman Supervisor:Associate Professor Abdullah S ipat, PhD
Call Number:FSAS 2001 39
Faculty or Institute:Faculty of Environmental Studies
ID Code:11506
Deposited By: Mohd Nezeri Mohamad
Deposited On:13 Jul 2011 09:45
Last Modified:15 May 2012 12:39

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