Improvement Of Molecular Methods For Detection Of Pathogenic Escherichia Coli.

Abdullah Al-Haj, Nagi Ahmed (2007) Improvement Of Molecular Methods For Detection Of Pathogenic Escherichia Coli. PhD thesis, Universiti Putra Malaysia.

[img] PDF
1522Kb

Abstract

Diarrhea is one of the leading causes of illnesses and death among children in developing countries, where an estimated 1.3 billion episodes and 4 to 10 million deaths occur each year in children below 5 years of age. The common pathogens of diarrhea are diarrheagenic Escherichia coli (DEC), Group A rotavirus, Shigella spp, Salmonella spp, Campylobacter, and Vibrio cholerae. Microbiological insights including phenotypic and genotypic characterisation are valuable approaches with application in management and prevention of diarrheal outbreaks by E. coli. In the present study, the Random Amplified polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates. Six out of 20 arbitrary primers namely, OPAE 4, 9, 10, 11, 12 and 18 produced DNA fingerprinting patterns providing the discriminatory power and the display of the potential epidemiological and diagnostic markers. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary

Item Type:Thesis (PhD)
Subject:Enteropathogenic Escherichia coli
Chairman Supervisor:Associate Professor Mariana Nor Shamsudin, PhD
Call Number:FPSK(P) 2007 3
Faculty or Institute:Faculty of Medicine and Health Science
ID Code:11279
Deposited By: Mohd Nezeri Mohamad
Deposited On:18 Jul 2011 01:52
Last Modified:17 Sep 2014 07:24

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 18 Jul 2011 01:52.

View statistics for "Improvement Of Molecular Methods For Detection Of Pathogenic Escherichia Coli."


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.