Characterisation Of Lactic Acid Bacteria And Bifidobacteria And Their Potential Application As A Probiotic Against Infant Diarrhea

Haque, K.M.formuzul (2000) Characterisation Of Lactic Acid Bacteria And Bifidobacteria And Their Potential Application As A Probiotic Against Infant Diarrhea. PhD thesis, Universiti Putra Malaysia.

[img] PDF


The major health promoting probiotic bacteria found in the human gut are of the genera Bifidobacterium and Lactobacillus. The main objectives of the present studies were to isolate, characterise and identify the suitable probiotic strains of Lactobacillus (LAB) and Bifidobacteria (BB) from faeces of breast fed infants which could be used as effective probiotic for the control of infant diarrheal diseases. From the isolation studies, the Lactobacillus was found to be Gram-positive, non-motile, short rods and catalase, nitrate, oxidase negative. The Bifidobacteria were Gram-negative, curved with characteristics of Y and V shapes. The high performance of liquid chromatography (HPLC) showed that almost all strains of BB produced more or less or equal amount of acetic and lactic acids. Based on the carbohydrate fermentation profile using API-CH-50 kits, out of 21 Lactobacillus, 16 species belong to the L. casei, four to L . brevis, one species to L. plantarum and one sub species casei. Twenty of Bifidobacteria were B. infantis species, where 13 belong to subspecies infantis, 7 were lacentis The antagonistic activities of LAB and BB were tested against ETEC E.coli 01 57:H7 and Salmonella typhimurium S-285 using double layered assay, results showed that strains LAB-3, 11, 21 and Bifi- l l , 1 9, 20 produced wider inhibition zone compared to others.

Item Type:Thesis (PhD)
Chairman Supervisor:Rokiah Mohd Yusof, PhD
Call Number:FPSK(P) 2000 2
Faculty or Institute:Faculty of Medicine and Health Science
ID Code:11269
Deposited By: Mohd Nezeri Mohamad
Deposited On:18 Jul 2011 02:10
Last Modified:08 Aug 2011 04:44

Repository Staff Only: Edit item detail

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.