Molecular Studies Of Vibrio Cholerae Strains Isolated From Local Outbreak

Wan Mohd. Zain, Wan Somarny (2003) Molecular Studies Of Vibrio Cholerae Strains Isolated From Local Outbreak. Masters thesis, Universiti Putra Malaysia.

[img] PDF


Cholera caused by toxigenic Vibrio cholerae is a major public health problem in developing countries. Epidemiology surveillance of cholera and comparative analysis of strains collected during outbreaks has demonstrated clonal diversity among epidemic strains and a continual emergence of new clones of toxigenic V. cholerae. In the present study, the Random Amplified polymorphic DNA (RAPD) technique was performed to study the clonal diversity on twenty isolates of V cholerae and five isolates of V. harveyi (as a comparison). Eighteen of V cholerae isolates belong to the Ogawa serotypes, while two isolates were Inaba and 0139 Bengal serotypes, respectively. Fourteen out of twenty random primers yielded clear and reproducible bands. From the RAPD banding profiles, the polymorphism rates of bands were much higher between V cholerae and V harveyi than those found among V cholerae isolates. According to the dendrogram generated from the RAPDistance software program,

Item Type:Thesis (Masters)
Chairman Supervisor:Associate Professor Mariana Nor Bt. Shamsudin, PhD
Call Number:FPSK(M) 2003 10
Faculty or Institute:Faculty of Medicine and Health Science
ID Code:11255
Deposited By: Mohd Nezeri Mohamad
Deposited On:18 Jul 2011 02:30
Last Modified:08 Aug 2011 04:56

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 18 Jul 2011 02:30.

View statistics for "Molecular Studies Of Vibrio Cholerae Strains Isolated From Local Outbreak "

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.