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Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus

Neela, Vasanthakumari (2000) Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus. Masters thesis, Universiti Putra Malaysia.

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Abstract

Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragment of the zot gene was successfully amplified in all the tested isolates. Asymmetry PCR was used as a preliminary detection method and later confirmed through the gene probe method to test for the presence of the zot gene. Consequently, the zot gene was successfully cloned in pCR 2.1 TOPO vector and sequenced, in which the sequences were 99% homologous to the gene bank sequences.

Item Type:Thesis (Masters)
Subject:Vibrio cholerae
Subject:Vibrionaceae
Subject:Gram negative bacteria
Chairman Supervisor:Dr. Mariana Nor Shamsudin
Call Number:FPSK(M) 2000 6
Faculty or Institute:Faculty of Medicine and Health Science
ID Code:11209
Deposited By: Mohd Nezeri Mohamad
Deposited On:18 Jul 2011 04:56
Last Modified:16 Aug 2011 02:19

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