The Effects Of Selected Pesticides On Cytochrome P450 System And Acetylcholinesterase Activity In Haruan, Channa Striatus

Yu, Tian (2000) The Effects Of Selected Pesticides On Cytochrome P450 System And Acetylcholinesterase Activity In Haruan, Channa Striatus. Masters thesis, Universiti Putra Malaysia.

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Abstract

Sebaran Sitokrom P-4S0 mikrosomal dikesan dalam organ haruan (Channa striatus), ikan air tawar tropika. Pengasingan mikrosom telah dilakukan berdasarkan tiga kaedah iaitu pengemparan-ultra pada 105,000 g selama 60 minit, pengemparan kelajuan-tinggi pada 40,000 g selama 90 minit dan teknik agregasi kalsium secara berturutan. Sitokrom P-450 hanya terdapat di dalam hati dan mikrosom usus, tetapi tidak di dalam organ lain seperti jantung, ginjal, limpa, insang dan otot. Karbon monoksida (CO) terturun sitokrom P-4S0 di dalam mikrosom hati menunjukkan penyerapan maksima spektrum pada 453 nm manakala di dalam usus mikrosom adalah pada 451 nm dalam mikrosom usus aman dengan menggunakan kedua - dua kaedah pengemparan-ultra dan kelajuan-tinggi. Pengemparan kelajuan-tinggi merupakan kaedah paling sesuai untuk digunakan di dalam pengasingan mikrosom kerana ia memberikan paras sitokrom p-4S0 yang paling tinggi iaitu sehingga 3.23 nm/g protein hepatik dan 2.24 nmol/g protein intestinal.

Item Type:Thesis (Masters)
Chairman Supervisor:Professor Madya Dr. Abdul Manan Mat Jais
Call Number:FPSK(M) 2000 1
Faculty or Institute:Faculty of Medicine and Health Science
ID Code:11204
Deposited By: Mohd Nezeri Mohamad
Deposited On:18 Jul 2011 05:02
Last Modified:12 Sep 2011 01:38

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