Application of a high density adsorbent in expanded bed adsorption of lipase from Burkholderia pseudomallei

Hor, Shee Yong and Beng, Ti Tey and Siew, Ling Hii and Mustapa Kamal, Siti Mazlina and Ariff, Arbakariya and Tau, Chuan Ling (2010) Application of a high density adsorbent in expanded bed adsorption of lipase from Burkholderia pseudomallei. African Journal of Biotechnology, 9 (2). pp. 203-216. ISSN 1684–5315

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Abstract

The application of STREAMLINE Direct HST adsorbent in expanded bed adsorption of lipase from Burkholderia pseudomallei was explored in this study. Scouting of optimum binding and elution condition was performed in batch binding mode. The addition of 0.2 M salt in acetate buffer (pH 5) during adsorption has increased the specificity and quantity of lipase binding onto the adsorbent. The addition of 0.4 M salt in phosphate buffer (pH 7) achieved the highest purification fold (2.5) in elution. The high density of the adsorbent allowed the EBA to be operated at linear velocity as high as 657 cm/h with feedstock containing 4.5% (w/v) wet biomass. The Richardson-Zaki correlation obtained for this EBA system at the presence of 4.5% (w/v) wet biomass is 5.14, a value closed to the laminar flow regime of 4.8, demonstrated that a stable bed is achieved under this operating condition. Meanwhile, a flow velocity of 343 cm/h with bed expansion of 3.2 gave highest dynamic binding capacity (4979.28 U/ml) and productivity (61.52 U/ml.min) for this EBA operation. It also demonstrated that biomass concentration up to 4.5% (w/v) wet weight showed slightly drop of sorption efficiency (0.82) compared to lower biomass concentration (0.94). Further increase of biomass concentration above 4.5% (w/v) wet weight has greatly decreased the equilibrium and dynamic capacity. Application of high density adsorbent tolerated

Item Type:Article
Keyword:Expanded bed adsorption, lipase, adsorbent, Burkholderia pseudomallei, high density.
Faculty or Institute:Faculty of Engineering
Publisher:Academic Journals
ID Code:11193
Deposited By: Anas Yahaya
Deposited On:25 Mar 2011 07:16
Last Modified:27 May 2013 07:48

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