In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9

Mohd Yusoff, Md Sabri and Saad, Mohd Zamri and Mutalib, Abdul Rahim and Israf Ali, Daud Ahmad and N., Muniandy (1999) In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9. Asia-Pacific Journal of Molecular Biology and Biotechnology, 7 (2). pp. 135-141. ISSN 0128-7451

Full text not available from this repository.

Abstract

The outer membrane proteins of Pasteurella haemolytica A2, A7 and A9 were subjected to SDS-PAGE and immunoblotting. The molecular weights of the polypeptide bands ranged between 33 to 97 kDa. The major polypeptide bands for P. haemolytica A2 were 33.4, 39.2 and 45 kDa while the minor polypeptide bands were 50, 58.7, 66.2, 84.7 and 97.4 kDa. Analysis of the outer membrane proteins of P. haemolytica A7 revealed two major protein bands of 33.4 and 45 kDa and three minor polypeptide of 40, 50 and 66.2 kDa. There were three major (33.4, 37.5 and 45 kDa) and one minor protein band (50 kDa) in the outer membrane proteins of P. haemolytica A9. There was one major protein band from each of the P. haemolytica A2, A7 and A9, which was unique to the respective serotype and appeared to represent the respective serotype. These were the 39.2 kDa band for P. haemolytica A2, the 40 kDa band for P. haemolytica A7 and the 37.5 kDa band for P. haemolytica A9. Following homologous immunoblot, all the serotypes showed pronounced antigenicity at the 30 kDa band. Heterologous immunoblot using the antiserum of P. haemolytica A2 did not reveal any antigenic band of P. haemolytica A9 but revealed antigenic bands at 30 and 31 kDa of P. haemolytica A7. Heterologous immunoblot using the antiserum of P. haemolytica A7 revealed antigenic band at 30 kDa of all the three serotypes while the antiserum of P. haemolytica A9 failed to reveal any common antigenic band between all three serotypes. Thus, the 30 kDa band of P. haemolytica A7 may be a suitable candidate for a sub-unit Vaccine against pneumonic pasteurellosis of sheep and goats.

Item Type:Article
Keyword:A1, Lipopolysaccharide, Specificity, Expression, Serotypes, Multocida, Cattle
Subject:Biotechnology - Research
Faculty or Institute:Faculty of Veterinary Medicine
ID Code:11043
Deposited By: Azizan Arshad
Deposited On:22 Mar 2011 03:28
Last Modified:22 Mar 2011 03:33

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 22 Mar 2011 03:28.

View statistics for "In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.