Kinetics and modelling of cell growth and substrate uptake in Centella asiatica cell culture

Omar, Rozita and Abdullah, M. A. and Hasan, M. A. and Mohammad, Rosfarizan and Mahmood, Maziah (2006) Kinetics and modelling of cell growth and substrate uptake in Centella asiatica cell culture. Biotechnology and Bioprocess Engineering, 11 (3). pp. 223-229. ISSN 1226-8372

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In this study, we have conducted kinetics and modelling studies ofCentella asiatica cell growth and substrate uptake, in an attempt to evaluate cell growth for a better understanding and control of the process. In our bioreactor cultivation experiment, we observed a growth rate of 0.18/day, a value only 20% higher than was seen in the shake flask cultivation trial. However, the observed maximum cell dry weight in the shake flask, 10.5 g/L, was 14% higher than was achieved in the bioreactor. Ninety seven percentage confidence was achieved via the fitting of three unstructured growth models; the Monod, Logistic, and Gompertz equations, to the cell growth data. The Monod equation adequately described cell growth in both cultures. The specific growth rate, however, was not effectively predicted with the Logistic and Gompertz equations, which resulted in deviations of up to 73 and 393%, respectively. These deviations in the Logistic and Gompertz models may be attributable to the fact that these models were developed for substrate-independent growth and fungi growth, respectively.

Item Type:Article
Keyword:kinetics, modelling, Centella asiatica, Monod, Logistic, Gompertz
Subject:Centella asiatica
Faculty or Institute:Faculty of Engineering
DOI Number:10.1007/BF02932034
ID Code:10824
Deposited By: Erni Suraya Abdul Aziz
Deposited On:17 Mar 2011 02:21
Last Modified:30 Oct 2011 05:12

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