Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)

Munari, Agus Sutanto (2001) Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.). Masters thesis, Universiti Putra Malaysia.

[img] PDF
1245Kb

Abstract

This study was carried out with the main objective of establishing somatic embryo production in papaya that may be used for further genetic improvement of the crop. The specific objectives were to induce somatic embryogenesis from immature zygotic embryos, to establish cell suspension culture of somatic embryos and to isolate and culture protoplasts from in vitro leaves. The first experiment studied the effect of growth regulators on the induction and regeneration of somatic embryos from immature zygotic embryos. The combinations of 2,4-dichlorophenoxyacetic acid (2,4-0) and 6-benzylaminopurine (BAP) at various concentrations were assessed in order to determine the best combination for somatic embryogenesis. The experiment was conducted in a Completely Randomized Design. The results showed that higher 2,4-D with or without BAP in the medium increased the percentage of somatic embryo formation and the number of somatic embryos per explant. MS medium supplemented with 5.0 mg/L 2,4-D promoted the highest percentage of somatic embryogenesis (74.55%), while the combinati on of 1.0 mg/L 2,4-0 and 0.01 mg/L BAP produced the highest percentage of callus formation (52. 58%). The highest number of somatic embryos per explant (66.61) was obtained when 5.0 mg/L 2,4-D and 0.01 mg/L BAP were added into MS medium. For germinati on of somatic embryos, the result was very inconsistent; nevertheless, the best treatment for plantlet formation was MS medium without growth hormone (NoBo). The second experiment was the establishment of cell suspensi on culture of somatic embryos. Four weeks after the transfer of somatic embryos into liquid MS medium containing 2.0 mg/L 2,4-0, pro-embryogenic masses (PEMs) were formed in the suspension. Maturation of embryos was achieved on transferring the heart-shaped embryos to liquid MS medium without growth regulator. Germination of somatic embryos occurred following the subculture of cotyledonary embryos from liquid MS medium with 0.2 mg/L BAP and 0.02 mg/L naphthalene acetic acid (NAA) to solid hormone-free MS medium.

Item Type:Thesis (Masters)
Subject:Papaya - Somatic embryogenesis - Case studies
Chairman Supervisor:Dr. Maheran Abdul Aziz
Call Number:FP 2001 16
Faculty or Institute:Faculty of Agriculture
ID Code:10559
Deposited By: Mohd Nezeri Mohamad
Deposited On:04 May 2011 04:03
Last Modified:04 May 2011 04:06

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 04 May 2011 04:03.

View statistics for "Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.