Improving a PCR – Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia

Khakvar, Reza and Sijam, Kamaruzaman and Wong, Mui Yun and Radu, Son and Thong, Kwai Lin (2008) Improving a PCR – Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia. American Journal of Agricultural and Biological Sciences, 3 (2). pp. 490-493. ISSN 1557-4989

[img] PDF
308Kb

Abstract

Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it’s considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various natural habits such as surface waters and different types of soils. Epidemiological and ecological studies on pathogen distribution in the environment are seriously hindered by the lack of efficient detection method especially when the concentration of the pathogen is either very low or is present in a latent, dormant or non-culturable state. With due attention to importance of R. solanacearum in Malaysia and several irreparable losses that every year caused by this bacterium, this is promted current study to develop a sensitive PCR-Based method to improve the detection of R. solanacearum in natural sources. We selected the previously reported primers (OLI1; OLI2; Y2; JE2) for their sensitivity and specificity detection of the bacterium in water and soil by a modification of PCR assay.

Item Type:Article
Faculty or Institute:Faculty of Agriculture
ID Code:851
Deposited By:INVALID USER
Deposited On:18 Dec 2008 12:49
Last Modified:26 Jul 2010 03:37

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 18 Dec 2008 12:49.

View statistics for "Improving a PCR – Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.