Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors
Mohd Shariff, Fairolniza and Leow, Thean Chor and Mukred, Abdul Daim and Salleh, Abu Bakar and Basri, Mahiran and Raja Abdul Rahman, Raja Noor Zaliha (2007) Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors. Journal of Basic Microbiology, 47 (5). pp. 406-412. ISSN 0233-111X; ESSN: 1521-4028
Official URL: http://onlinelibrary.wiley.com/wol1/doi/10.1002/jo...
A thermophilic bacterium, Bacillus sp. strain L2 was isolated from a hot spring in Perak, Malaysia. An extracellular lipase activity was detected through plate and broth assays at 70 °C after 28 h of incubation. The L2 lipase production was growth dependent as revealed by a number of factors affecting the secretion of extracelullar lipase. As for nutritional factors, casamino acids, trehalose, Ca2+ and Tween 60 were found to be more effective for lipase production. The optimum physical condition for L2 lipase production was obtained at 70 °C after 28 h of cultivation time, at pH 7.0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa.
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