Ngo, L. T. A. and Pham, T. L. and Le, V. V. M. (2008) Purification of Endopolygalacturonase from Submerged Culture of Aspergillus awamori L1 Using a Two-step Procedure: Enzyme Precipitation and Gel Filtration. International Food Research Journal, 15 (2). pp. 135-140. ISSN 1985-4668
The purification of extracellular endopolygalacturonase (endo-PG) from the filtrate of Aspergillus awamori L1 submerged culture was carried out using a two-step procedure: enzyme precipitation by organic solvents, polymers or salts; and gel filtration using Sephadex G-75 column. The results show that ammonium sulfate was the suitable precipitant in the two-step procedure for endo-PG purification. Firstly, ammonium sulfate was added to the crude enzyme solution with the concentration of 47.2% (w/v) for endo-PG precipitation. The precipitates were then isolated and redissolved in a buffer solution to prepare for the gel filtration step. Consequently, the purification factor achieved 30.4-fold and the endo-PG recovery yield obtained 68.60% in comparison to the crude enzyme solution.
|Keyword:||Aspergillus awamori L1, precipitation, gel filtration, submerged fermentation, endopolygalacturonase|
|Faculty or Institute:||Faculty of Food Science and Technology|
|Deposited By:||Yusfauhannum Mohd Yunus|
|Deposited On:||26 Nov 2008 03:32|
|Last Modified:||30 Mar 2010 15:30|
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