Typing of Erwinia Chrysanthemi Isolated from Josapine Pineapple in Malaysia Using Antimicrobial Susceptibility, Plasmid Profiles, ERIC-PCR and RFLP Analysis

Abd Mutalib, Sahilah and Laboh, Rozeita and Md Shah, Umi Kalsum and Radu, Son (2008) Typing of Erwinia Chrysanthemi Isolated from Josapine Pineapple in Malaysia Using Antimicrobial Susceptibility, Plasmid Profiles, ERIC-PCR and RFLP Analysis. International Food Research Journal, 15 (3). pp. 273-280. ISSN 1985-4668

[img] PDF
218Kb

Official URL: http://www.ifrj.upm.edu.my/15%20(3)%202008/04.%20Sahilah%20A.%20M.pdf

Abstract

Ninety one leaf samples of Josapine pineapple cultivar (Kelantan, n=8; Pahang, n=20; Perak, n=11; Sabah, n=15; Johor, n=37) showing symptoms of heart rot disease were collected to determine the incidence of Erwinia chrysanthemi. Sixteen strains of E. chrysanthemi were isolated from 13 leaf samples from Pahang (n=4), Sabah (n=2) and Johor (n=7). All of the E. chrysanthemi strains displayed resistance to bacitracin with two strains showing resistance to sulfamethoxazole. None of the E. chrysanthemi strains were resistant toward ampicillin, carbenicillin, cephalothin, ceftriaxone, cefuroxime, gentamicin, kanamycin, nalidixic acid, penicillin G, streptomycin and tetracycline. All of the E. chrysanthemi strains were plasmidless. The dendrogram generated from the ERIC-PCR fingerprinting showed that the E. chrysanthemi strains formed 4 clusters and 7 single isolates at 80% similarity level. The restriction fragment length polymorphism (RFLP) analysis for 16 strains of E. chrysanthemi with HinfI and HaeIII endonuclease, 2 and 4 restriction profiles were obtained, respectively. The combinations of the four techniques were able to differentiate the 16 E. chrysanthemi strains into 14 genome types, suggesting a wide diversity of strains examined. ERICPCR fingerprinting method is found to be more discriminating and useful for the determination of the E. chrysanthemi strains relatedness.

Item Type:Article
Faculty or Institute:Faculty of Food Science and Technology
ID Code:738
Deposited By: Yusfauhannum Mohd Yunus
Deposited On:25 Nov 2008 17:41
Last Modified:27 May 2013 06:50

Repository Staff Only: item control page


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.