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Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor


Citation

Lo, Seong Koon and Cheong, Ling Zhi and Arifin, Norlelawati and Tan, Chin Ping and Long, Kamariah and Yusoff, Mohd Suria Affandi and Lai, Oi Ming (2007) Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor. Journal of Agricultural and Food Chemistry, 55 (14). pp. 5595-5603. ISSN 0021-8561

Abstract / Synopsis

Diacylglycerol (DAG) and triacylglycerol (TAG) as responses on optimization of DAG production using a dual response approach of response surface methodology were investigated. This approach takes the molecular equilibrium of DAG into account and allows for the optimization of reaction conditions to achieve maximum DAG and minimum TAG yields. The esterification reaction was optimized with four factors using a central composite rotatable design. The following optimized conditions yielded 48 wt % DAG and 14 wt % TAG: reaction temperature of 66.29 °C, enzyme dosage of 4 wt %, fatty acid/glycerol molar ratio of 2.14, and reaction time of 4.14 h. Similar results were achieved when the process was scaled up to a 10 kg production in a pilot packed-bed enzyme reactor. Lipozyme RM IM did not show any significant activity losses or changes in fatty acid selectivity on DAG synthesis during the 10 pilot productions. However, lipozyme RM IM displayed higher selectivity toward the production of oleic acid-enriched DAG. The purity of DAG oil after purification was 92 wt %.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: 10.1021/jf0706676
Publisher: American Chemical Society
Keywords: Diacylglycerol, dual response, optimization, response surface methodology, packed-bed reactor, lipozyme RM IM, Rhizomucor miehei lipase, esterification, pilot plant
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Jun 2010 09:21
Last Modified: 05 Oct 2015 16:33
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1021/jf0706676
URI: http://psasir.upm.edu.my/id/eprint/7303
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