Isolation of salinity tolerant genes from the mangrove plant, Bruguiera cylindrica by using suppression subtractive hybridization (SSH) and bacterial functional screening

Wong, Yeen Yee and Ho, Chai Ling and Nguyen, Phuoc Dang and Teo, Swee Sen and Harikrishna, Jennifer Ann and Abdul Rahim, Raha and Wong, Michael C. V. L. (2007) Isolation of salinity tolerant genes from the mangrove plant, Bruguiera cylindrica by using suppression subtractive hybridization (SSH) and bacterial functional screening. Aquatic Botany, 86 (2). pp. 117-122. ISSN 0304-3770

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.aquabot.2006.09.009

Abstract

In this study, we have identified and isolated 126 salinity tolerant cDNAs from the root of a mangrove plant, Bruguiera cylindrica (L.) Blume by using suppression subtractive hybridization (SSH) and bacterial functional screening. Sequencing of 51 subtracted cDNA clones that were differentially expressed in the root of B. cylindrica exposed to 20 parts per thousand (ppt) NaCl water revealed 10 tentative unique genes (TUGs) with putative functions in protein synthesis, storage and destination, metabolism, intracellular trafficking and other functions; and 9 unknown proteins. Meanwhile, the 75 cDNA sequences of B. cylindrica that conferred salinity tolerance to Escherichia coli consisted of 29 TUGs with putative functions in transportation, metabolism and other functions; and 33 with unknown functions. Both approaches yielded 42 unique sequencess that have not been reported else where to be stress related and might provide further understanding of adaptations of this plant to salinity stress.

Item Type:Article
Keyword:Mangrove, Bacterial functional screening, Bruguiera cylindrica, Salinity tolerance, Sequence analysis, Suppression subtractive hybridization (SSH)
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
DOI Number:10.1016/j.aquabot.2006.09.009
ID Code:7262
Deposited By: Erni Suraya Abdul Aziz
Deposited On:14 Jun 2010 07:22
Last Modified:14 Jun 2010 07:25

Repository Staff Only: item control page


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.