Optimization of Environmental and Nutritional Conditions for the Production of Alkaline Protease by a Newly Isolated Bacterium Bacillus cereus Strain 146

Shafee, Norazizah and Aris, Sayangku Norariati and Raja Abdul Rahman, Raja Noor Zaliha and Basri, Mahiran and Salleh, Abu Bakar (2005) Optimization of Environmental and Nutritional Conditions for the Production of Alkaline Protease by a Newly Isolated Bacterium Bacillus cereus Strain 146. Journal of Applied Sciences Research, 1 (1). pp. 1-8. ISSN 1816-157X

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Abstract

Optimization of the growth conditions for maximum growth rate and alkaline protease production was carried out using the newly isolated mesophilic bacterium Bacillus cereus strain 146. The bacterium produced protease at maximum rate after 48 h of incubation at 37°C with agitation speed of 170 rpm and 4% (v/v) starter culture. The best carbon and organic nitrogen sources for this bacterium were glucose and beef extract, respectively. While the most effective inorganic nitrogen sources were urea and lysine. Supplementation of the culture medium with Mn2+ improved the protease production substantially. Under these conditions B. cereus strain 146 was found to produce alkaline protease at a maximum rate of approximately 2.0 μg/mL/min.

Item Type:Article
Keyword:Alkaline protease, Bacillus cereus, environmental factors, nutritional conditions
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:INSInet Publication
ID Code:7088
Deposited By: Erni Suraya Abdul Aziz
Deposited On:09 Jun 2010 03:30
Last Modified:27 Jul 2010 09:24

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