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Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53


Citation

Yan, See Wan and Rahmat, Asmah (2017) Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53. International Food Research Journal, 24 (3). pp. 1331-1337. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Practiced as folk medicine since ancient times, bilimbi (Averrhoa bilimbi) is commonly consumed and widely cultivated in Malaysia. In search for naturally occurring anticancer agents, a potential fruit extract was found to exert anticancer properties in vitro without any cytotoxic effect on normal cells. This study investigated the anti-proliferative effect and underlying cell death pathway induced by bilimbi ethanolic extract on human non-hormone-dependent breast cancer cell line, MDA-MB-231. Anticancer potential of bilimbi extract was conducted by investigating the in vitro growth inhibitory effect, DNA fragmentation, cell cycle progression and anti-proliferation assay. Release of caspases, cytochrome c and apoptotic proteins were demonstrated to determine the mechanism of cell death pathway. Findings revealed that bilimbi inhibited growth of MDA-MB-231 cells through the induction of apoptosis mediated by cell cycle arrest at G0/G1 checkpoint. Released of cytochrome c coupled with up-regulation of caspase-3/7, caspase-9 and Bax pro-apoptotic proteins in addition to down-regulation of dysfunctional p53 and Bcl-2 anti-apoptotic proteins implied that bilimbi induced a p53-independent mitochondrial apoptosis pathway in MDA-MB-231. These results suggest that bilimbi could induce tumor cell anti-proliferation through apoptosis. As a natural product, bilimbi could be a promising anticancer agent and an inexpensive approach to cancer chemoprevention strategy.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Averrhoa bilimbi; MDA-MB-231; Apoptosis; G0/G1 cell cycle arrest
Depositing User: Nabilah Mustapa
Date Deposited: 12 Jan 2018 04:44
Last Modified: 12 Jan 2018 04:44
URI: http://psasir.upm.edu.my/id/eprint/58363
Statistic Details: View Download Statistic

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