Comparative evaluations of the processed bovine tunica vaginalis implant in a rat model

Yagoub Mohamed, Abdel Hafeez and Abu Bakar Zakaria, Md Zuki and Mohamed Yusof, Loqman and Mohamed Mustapha, Noordin and Yusof, Norimah (2005) Comparative evaluations of the processed bovine tunica vaginalis implant in a rat model. Anatomical Science International, 80 (4). pp. 181-188. ISSN 1447-6959

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1111/j.1447-073X.2005.00111.x

Abstract

The aim of the present study was to evaluate the lyophilized and glycerolized bovine parietal tunica vaginalis for repair of a full-thickness abdominal wall defect and to compare their effectiveness with expanded polytetrafluoroethylene (ePTFE) Mycro Mesh (Gore-Tex® MYCRO MESH®; Gore and Designs, W. L. Gore & Associates, Flagstaff, AZ, USA) in a rat model. Fresh bovine parietal tunica vaginalis sacs collected from an abattoir were processed and preserved by freeze-drying and by using 99.5% glycerol. Full-thickness abdominal wall defects (3 × 2.5 cm) created surgically in 90 male Sprague-Dewaly rats (300–400 g) were repaired with the same size of lyophilized, glycerolized or ePTFE Mycro Mesh with 30 rats in each group. Six rats from each group were killed at post-implantation intervals of 1, 3, 6, 9 and 18 weeks for macroscopic, microscopic and tensiometric evaluations. All rats survived the procedure, except for one rat in the ePTFE Mycro Mesh group. Implants of bovine origin were gradually resorbed and replaced with recipient fibrous tissue, whereas the mesh implant was encapsulated with fibrous tissue and remained without any marked changes throughout the study period. Adhesions between the implant and underlying visceral organs were encountered in 10, 6.6 and 3.3% of rats implanted with lyophilized, glycerolized or ePTFE Mycro Mesh, respectively. Foreign body giant cells and calcification were demonstrated in fibrous capsule and mesh matrix, respectively, in rats implanted with ePTFE Mycro Mesh. Neither of these characteristics were observed in rats implanted with processed bovine tunica vaginalis. Macrophages engorged with lipofuchsin pigments were observed in the recipient tissue that replaced the implants of bovine origin at 3–18 weeks post-implantation. There were no significant (P > 0.05) differences in total mean values of healing tensile strength, load at break and Young’s modulus of elasticity between the three implant groups. The results of the present study encourage further investigation into the use of processed bovine parietal tunica vaginalis in clinical practice.

Item Type:Article
Keyword:abdominal defect, bovine tunica vaginalis, glycerol, healing strength
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Springerlink
DOI Number:10.1111/j.1447-073X.2005.00111.x
ID Code:5806
Deposited By: Azizan Arshad
Deposited On:06 May 2010 08:44
Last Modified:19 Aug 2010 05:56

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 06 May 2010 08:44.

View statistics for "Comparative evaluations of the processed bovine tunica vaginalis implant in a rat model"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.