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Dual regulation of cell death and cell survival upon induction of cellular stress by Isopimara-7, 15-Dien-19-Oic acid in cervical cancer, HeLa cells in vitro

Abu, Nadiah and Swee, K Yeap and Mat Pauzi, Ahmad Z and Akhtar, Muhammad Nadeem and Zamberi, Nur Rizi and Ismail, Jamil and Zareen, Seema and Alitheen, Noorjahan Banu (2016) Dual regulation of cell death and cell survival upon induction of cellular stress by Isopimara-7, 15-Dien-19-Oic acid in cervical cancer, HeLa cells in vitro. Frontiers in Pharmacology, 7 (89). pp. 1-11. ISSN ESSN: 1663-9812

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Abstract

The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways.

Item Type:Article
Keyword:HeLa; Isopimara-7, 15-dien-19-oic acid; Fritillaria imperialis; Antioxidant
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher:Frontiers Research Foundation
DOI Number:10.3389/fphar.2016.00089
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3389/fphar.2016.00089
ID Code:55569
Deposited By: Mohd Hafiz Che Mahasan
Deposited On:14 Aug 2017 18:21
Last Modified:14 Aug 2017 18:21

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