Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit

Citation

Alsharif, A. H. K. H. and Hamzah, Hazilawati and Shaari, Mohd Rosly and Mohamed Mustapha, Noordin and Sithambaram, Shanmugavelu and Haron, Abd Wahid and K., Musaddim and A. M., Nabil (2012) Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 379-381).

Abstract

This study was conducted to determine erythrocyte glutathione peroxidase (GSH-Px) activity, in pregnant Boer does supplemented daily with and without dried Morinda citrifolia fruit, analysed using two different methods; a manual colourimetric method (direct method) and a commercial GSH-Px ELISA kit. The does were supplemented with M. citrifolia fruit started from third trimester of pregnancy until 2.5 months post partum. Results showed that GSH-Px analysed using the direct method produced insignificant differences between the groups throughout the experimental period, while the commercial ELISA kit produced some significant differences between the groups during the experiment. These indicate that erythrocyte GSH-Px activity is more stable analysed using the commercial kit and hence provides more accurate results compared to the direct method.

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Additional Metadata

Item Type:	Conference or Workshop Item (Poster)
Divisions:	Faculty of Veterinary Medicine
Keywords:	Gluthathione peroxidase; Dried Morinda citrifolia fruit; Pregnant Boer goats
Depositing User:	Nabilah Mustapa
Date Deposited:	02 Feb 2017 05:02
Last Modified:	02 Feb 2017 05:02
URI:	http://psasir.upm.edu.my/id/eprint/50234
Statistic Details:	View Download Statistic

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