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Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro

Abu, Nadiah and Akhtar, Muhammad Nadeem and Yeap, Swee Keong and Lim, Kian Lam and Ho, Wan Yong and Abdullah, Mohd Puad and Ho, Chai Ling and Omar, Abdul Rahman and Ismail, Jamil and Mohammed Alitheen, Noorjahan Banu (2016) Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro. BMC Complementary and Alternative Medicine, 16 (1). art. no. 86. pp. 1-14. ISSN 1472-6882

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Official URL: http://link.springer.com/article/10.1186/s12906-01...

Abstract

Background: The kava-kava plant (Piper methysticum) is traditionally consumed by the pacific islanders and has been linked to be involved in several biological activities. Flavokawain B is a unique chalcone, which can be found in the roots of the kava-kava plant. In this study, the operational mechanism of the anti-cancer activity of a synthetic Flavokawain B (FKB) on two breast cancer cell lines, MCF-7 and MDA-MB231 was investigated. Method: Several in vitro assays were attempted such as MTT, flow cytometry of cell cycle analysis, annexin V analysis, and JC-1 analysis to detect apoptosis. Moreover, in vitro metastasis assays were also performed such as transwell migration assay, invasion assay, rat aorta ring and HUVEC tube formation. Molecular analysis of related genes and proteins were conducted using real-time PCR and proteome profiler analysis. Results: Based on our results, apoptosis was induced when both MCF-7 and MDA-MB231 were treated with FKB. A significant G2/M arrest was seen in MDA-MB231 cells. Additionally, FKB also inhibited the in vitro migration and invasion in MDA-MB231 cells in a dose dependent manner. Moreover, FKB can be a potential inhibitor in angiogenesis as it suppressed the formation of vessels in HUVEC cells as well as in the ex-vivo rat aortic ring assay. Conclusion: Our findings suggested that FKB also regulated several receptor tyrosine kinases. Overall, FKB is not only a potential candidate to be an anti-cancer agent, but as an anti-metastatic agent as well.

Item Type:Article
Keyword:Breast cancer; Flavokawain; Metastasis; Proliferation; Tyrosine kinase
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher:BioMed Central
DOI Number:10.1186/s12906-016-1046-8
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1186/s12906-016-1046-8
ID Code:47474
Deposited By: Nabilah Mustapa
Deposited On:20 May 2016 09:53
Last Modified:20 May 2016 09:53

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