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Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene


Citation

Mohamad Wali, Haryati Shila and Ideris, Aini and Omar, Abdul Rahman and Bejo, Mohd Hair (2013) Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 111-112).

Abstract

The significance of the role of mitochondria incontending viral infection is gaining worldwide interest. Its ability to elicit molecular signals to other immune components enables new perspective in the development of novel antiviral agents. This study explored the possibility of isolating the mitochondrial antiviral signaling (MAVS) gene from chicken tissue and cloning it into cloning vector. Prior to cloning, RNA extraction was carried out from chicken samples and converted to cDNA. Polymerase chain reaction (PCR) amplified product was ligated to the vector pJET1.2. The resulting plasmid construct pJET1.2/CARDIF was then transformed into competent cells and later sequenced and analysed by restriction enzyme analysis. BLAST analysis of the construct yields 99% similarity with Gallus gallus CARDIF mRNA gene.


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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Faculty of Veterinary Medicine, Universiti Putra Malaysia
Keywords: Mitochondrial antiviral signaling gene; Antiviral response; Type I interferon
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Nov 2015 06:23
Last Modified: 28 Jan 2016 01:39
URI: http://psasir.upm.edu.my/id/eprint/41408
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