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Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin


Citation

Hong, Chia Yean and Wong, Nyet Kui and Abdullah, Maha (2015) Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin. Asian Pacific Journal of Allergy and Immunology, 33 (1). pp. 26-32. ISSN 0125-877X

Abstract

Background: Tamm-Horsfall glycoprotein (THP) and uromodulin are the most abundant glycoproteins in non-pregnant women’s/men’s and pregnant women’s urine, respectively. However, the bioactivities of these glycoproteins are still unclear. Objective: To evaluate the immunomodulatory properties of THP and uromodulin on human peripheral blood mononuclear cells (PBMC). Methods: THP and uromodulin isolated with diatomaceous earth filtration were subjected to several bioassays, such as MTS viability assay, immunophenotyping and cytokine analysis. Results: MTS viability assay and immunophenotyping analysis showed that uromodulin has greater inhibitory activities in suppressing PBMC viability and the percentage of CD4+ T helper cells and CD8+ cytotoxic T cells, compared to that of THP. In cytokine analysis, THP tended to induce pro-inflammatory cytokines such as IL-1β, TNF and Th1 cytokine IFN-γ; while uromodulin only induced IL-1β and suppressed both Th1 cytokine IFN-γ and Th2 cytokine IL-10. Conclusion: These results demonstrate that uromodulin has greater immunosuppressive activities and lower inductive property in relation to activation of immune cells, which provides a more tolerant environment for the developing fetus.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Allergy, Asthma and Immunology Association of Thailand
Keywords: Cytokine; Immunophenotyping; Immunosuppressive; MTS assay; Tamm-Horsfall glycoprotein; Uromodulin
Depositing User: Nabilah Mustapa
Date Deposited: 29 Dec 2015 12:26
Last Modified: 29 Dec 2015 12:26
URI: http://psasir.upm.edu.my/id/eprint/37938
Statistic Details: View Download Statistic

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