Citation
Thanarajoo, Sathis Sri and Kong, Lih Ling and Kadir, Jugah and Lau, Wei Hong and Vadamalai, Ganesan
(2014)
Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP).
Journal of Virological Methods, 202.
pp. 19-23.
ISSN 0166-0934; ESSN: 1879-0984
Abstract
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60. min at 60. °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix after the addition of fluorescent reagent, and a laddering pattern band on 2% agarose gel electrophoresis. Conventional RT-PCR with LAMP primers produced amplicons with a sequence identical to the 297-nt CCCVd oil palm variant with the primers being specific for CCCVd and not for other viroids such as PSTVd and CEVd. RT-LAMP was found to be rapid and specific for detecting oil palm CCCVd.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture Institute of Tropical Agriculture |
| DOI Number: | https://doi.org/10.1016/j.jviromet.2014.02.024 |
| Publisher: | Elsevier |
| Keywords: | Coconut cadang-cadang viroid; Oil palm; RT-LAMP |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 02 Jun 2015 12:10 |
| Last Modified: | 01 Dec 2015 02:26 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jviromet.2014.02.024 |
| URI: | http://psasir.upm.edu.my/id/eprint/37013 |
| Statistic Details: | View Download Statistic |
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