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Desiccation tolerance in Phaleria macrocarpa embryonic axes

Ahmed Asrity, S. M. and Tsan, Fui Ying and Ding, Phebe and Syed Aris, Syaripah Ruzaini (2014) Desiccation tolerance in Phaleria macrocarpa embryonic axes. Sains Malaysiana, 43 (10). pp. 1471-1475. ISSN 0126-6039

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Abstract

Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a horizontal laminar air flow cabinet. They were then desiccated under aseptic condition for periods ranging from 0-8 h. For each desiccation treatment, embryonic axes were drawn randomly for the determination of MC according to ISTA, electrolyte leakage and proliferation on Murashige and Skoog (MS) media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The results obtained from this study indicated that the embryonic axes could tolerate dehydration down to 13.6% with desiccation for 8 h while retaining relatively high viability of 76.7%. This was supported by only gradual increment of electrolyte leakage with the desiccated embryonic axes. All non-desiccated embryonic axes with MC of 52.5% were capable to grow into normal plantlets in vitro but dehydration to MC of 36.0% and further down to 13.6% generally resulted in callus formation with up to 16.7% of the embryonic axes while at least 60.0% of the other embryonic axes were still capable to proliferate as normal plantlets in vitro.

Item Type:Article
Keyword:Callus; Electrolyte leakage; Moisture content; Seed; Survival
Faculty or Institute:Faculty of Agriculture
Publisher:Penerbit Universiti Kebangsaan Malaysia
ID Code:36990
Deposited By: Nabilah Mustapa
Deposited On:26 May 2015 20:39
Last Modified:26 Aug 2016 16:54

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