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Application of PCR-ELISA in molecular diagnosis


Citation

Sue, Mei Jean and Yeap, Swee Keong and Omar, Abdul Rahman and Tan, Sheau Wei (2014) Application of PCR-ELISA in molecular diagnosis. BioMed Research International, 2014. art. no. 653014. pp. 1-6. ISSN 2314-6133; ESSN: 2314-6141

Abstract

Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.


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Official URL or Download Paper: http://www.hindawi.com/journals/bmri/2014/653014/

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.1155/2014/653014
Publisher: Hindawi Publishing Corporation
Keywords: PCR-ELISA; Polymerase chain reaction; Method
Depositing User: Nabilah Mustapa
Date Deposited: 18 Aug 2015 12:25
Last Modified: 26 Jan 2016 07:50
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2014/653014
URI: http://psasir.upm.edu.my/id/eprint/36517
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