Simple Search:

Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2


Citation

Ahmad Kamarudin, Nor Hafizah and Raja Abdul Rahman, Raja Noor Zaliha and Mohamad Ali, Mohd Shukuri and Leow, Thean Chor and Basri, Mahiran and Salleh, Abu Bakar (2014) Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2. Molecular Biotechnology, 56 (8). pp. 747-757. ISSN 1073-6085; ESSN: 1559-0305

Abstract / Synopsis

Terminal moieties of most proteins are long known to be disordered and flexible. To unravel the functional role of these regions on the structural stability and biochemical properties of AT2 lipase, four C-terminal end residues, (Ile–Thr–Arg–Lys) which formed a flexible, short tail-like random-coil segment were targeted for mutation. Swapping of the tail-like region had resulted in an improved crystallizability and anti-aggregation property along with a slight shift of the thermostability profile. The lipolytic activity of mutant (M386) retained by 43 % compared to its wild-type with 18 % of the remaining activity at 45 °C. In silico analysis conducted at 25 and 45 °C was found to be in accordance to the experimental findings in which the RMSD values of M386 were more stable throughout the total trajectory in comparison to its wild-type. Terminal moieties were also observed to exhibit large movement and flexibility as denoted by high RMSF values at both dynamics. Variation in organic solvent stability property was detected in M386 where the lipolytic activity was stimulated in the presence of 25 % (v/v) of DMSO, isopropanol, and diethyl ether. This may be worth due to changes in the surface charge residues at the mutation point which probably involve in protein–solvent interaction.


Download File

[img]
Preview
PDF (Abstract)
Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2.pdf

Download (51kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: 10.1007/s12033-014-9753-1
Publisher: Springer
Keywords: C-terminal region; Deletion; Crystallizability; Thermostability; Organic solvent stability
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 11 Feb 2016 16:42
Last Modified: 26 Sep 2016 11:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12033-014-9753-1
URI: http://psasir.upm.edu.my/id/eprint/35925
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item