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Optimization of lipase-catalyzed synthesis of N-trans-feruloyltyramine using response surface methodology (RSM)


Citation

Basri, Mahiran and Alrub, Mohammad Abu and Abd Malek, Emilia and Alang Ahmad, Shahrul Ainliah and Salleh, Abu Bakar and Abdul Rahman, Mohd Basyaruddin (2014) Optimization of lipase-catalyzed synthesis of N-trans-feruloyltyramine using response surface methodology (RSM). Chemical Engineering Communications, 201 (12). pp. 1582-1592. ISSN 0098-6445; ESSN: 1563-5201

Abstract

Enzymatic synthesis of N-trans-feruloyltyramine amide was optimized by response surface methodology (RSM) using 4-hydroxy-3-methoxycinnamic acid and tyramine hydrochloride in a one-step lipase catalyzed reaction using Lipozyme TL IM. Response surface methodology (RSM) based on five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interaction of synthesis, reaction time (24–96 h), temperature (30°–50°C), amount of enzyme (50–500 mg, 12.5–125.0 IUN), and substrate molar ratio (cinnamic acid:tyramine HCl) 1:1–8:1 mmol on the percentage yield of N-trans-feruloyltyramine amide. The optimum conditions derived via RSM were: reaction time 52 h, temperature 43°C, amount of enzyme 260 mg (65.0 IUN), and substrate molar ratio (cinnamic acid:tyramine HCl) 6.2:1. The actual experimental yield was 96.3% under optimum conditions, which compared well to the maximum predicted value of 97.2%.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1080/00986445.2013.821060
Publisher: Taylor & Francis
Keywords: Cinnamic acid; Enzymatic synthesis; Lipozyme; N-trans-feruloyltyramine; RSM; Tyramine HCL
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 23 Dec 2015 00:44
Last Modified: 23 Dec 2015 00:44
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/00986445.2013.821060
URI: http://psasir.upm.edu.my/id/eprint/34773
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