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Optimization of enzymatic synthesis of 3-O-B-D-glucopyranoside betulinic acid by Novozyme-435

Abdu, Hamisu and Ahmad@Amat, Faujan and Basri, Mahiran and Ismail, Intan Safinar and Abdul Rahman, Mohd Basyaruddin (2014) Optimization of enzymatic synthesis of 3-O-B-D-glucopyranoside betulinic acid by Novozyme-435. Asian Journal of Research in Chemistry, 7 (7). 640-643 . ISSN 0974-4169; ESSN: 0974-4150

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Abstract

The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69 % and the predicted value was 89.05 %.

Item Type:Article
Keyword:Enzymes; Enzymatic synthesis; 3-O-β-D-glucopyranoside betulinic acid; Novozyme-435; Optimization
Faculty or Institute:Faculty of Science
Publisher:A & V Publications
ID Code:34770
Deposited By: Nurul Ainie Mokhtar
Deposited On:22 Dec 2015 19:53
Last Modified:22 Dec 2015 19:53

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