A Protocol for Efficient Plantlet Regeneration from Leaf Derived Callus of Lablab Bean (Lablab purpureus var. lignosus (L) prain)

Thiruvengadam, Muthu and Jayabalan, Narayanasamypillai (2003) A Protocol for Efficient Plantlet Regeneration from Leaf Derived Callus of Lablab Bean (Lablab purpureus var. lignosus (L) prain). Pertanika Journal of Tropical Agricultural Science, 26 (1). pp. 35-40. ISSN 1511-3701

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Abstract

An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (IAA, 2, 4-D and NAA) and cytokinin (RAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and RAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of RAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields.

Item Type:Article
Keyword:Lablab purpureus, multiple shoot induction, organogenesis, tissue culture
Publisher:Universiti Putra Malaysia Press
ID Code:3460
Deposited By: Farhana Yusof
Deposited On:26 Nov 2009 04:02
Last Modified:27 May 2013 07:08

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