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A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)


Citation

Thiruvengadam, Muthu and Jayabalan, Narayanasamypillai (2003) A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain). Pertanika Journal of Tropical Agricultural Science, 26 (1). pp. 35-40. ISSN 1511-3701

Abstract / Synopsis

An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (IAA, 2, 4-D and NAA) and cytokinin (RAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and RAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of RAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields.


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Additional Metadata

Item Type: Article
Publisher: Universiti Putra Malaysia Press
Keywords: Lablab purpureus; Multiple shoot induction; Organogenesis; Tissue culture
Depositing User: Farhana Yusof
Date Deposited: 26 Nov 2009 12:02
Last Modified: 11 Sep 2015 10:54
URI: http://psasir.upm.edu.my/id/eprint/3460
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